Localization of autophagy proteins using immunofluorescence, confocal microscopy and western blot techniques

Cabanu, Sharon (a.a. 2016/2017) Localization of autophagy proteins using immunofluorescence, confocal microscopy and western blot techniques. Tesi di Laurea in Biotecnologie (L-2), Università degli studi di Sassari, relatore Sciola Gian Luigi, pp. 15. [Tesi di Laurea triennale]

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Abstract

Autophagy is a self-digesting pathway by which old proteins and organelles are removed and recycled. The cytoplasmic organelles and proteins are wrapped by an isolation endomembrane that matures into an autophagosome. This undergoes a multistep maturation process including fusion with endosomes and lysosomes, and in which the engulfed cytoplasm is degraded by the lysosomal hydrolases. Beclin 1 is involved in the early steps of autophagosome formation. It forms a complex with Vps34, a kinase that produces phosphatidyl inositol 3-phosphage (PI3P) necessary for the recruitment of downstream autophagy proteins. The precise role of Beclin 1 (mammalian orthologue of Atg6) localization in the cell is still unknown. Atg69 is a protein involved in autophagosome formation. ULK1 is a protein kinase also involved in autophagosome formation. It phosphorylates Beclin 1 and regulates the activity and traffic of Atg9.

Tipologia di tesi: Tesi di Laurea triennale
Autore: Cabanu, Sharon
Relatore: Sciola, Gian Luigi
Disciplina MIUR: Area 05 - Scienze biologiche > BIO/06 ANATOMIA COMPARATA E CITOLOGIA
Struttura: Dipartimento di Scienze Biomediche
Corsi di Laurea: Biotecnologie (L-2)
Anno Accademico: 2016/2017
Sessione: Straordinaria
Parole Chiave: Beclin 1, Atg9, MEF-ULK cell line, ULK 1/2
Codice ID dell'EPrint: 1057
Data di Deposito: 28 Feb 2018 15:32
Tipo di tesi: Sperimentale
URI: http://unisslaurea.uniss.it/id/eprint/1057

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